Abstract #2769
Hyperpolarized Ketone Body Metabolism in the Perfused Rat Heart
Damian J Tyler 1 , Angus Z Lau 1,2 , and Daniel R Ball 1
1
Dept. of Physiology, Anatomy & Genetics,
University of Oxford, Oxford, Oxfordshire, United
Kingdom,
2
Dept.
of Cardiovascular Medicine, University of Oxford,
Oxford, Oxfordshire, United Kingdom
Hyperpolarization of [1-
13
C]pyruvate provides
a unique insight into various aspects of carbohydrate
metabolism. However, alterations in the metabolic
pathways of other key fuel molecules (e.g. fatty acids
and ketone bodies) are a common feature of many
diseases. This work aimed to produce a reliable method
to hyperpolarize the ketone bodies, acetoacetate and β-hydroxybutyrate,
and to investigate their metabolism in the perfused rat
heart. Following successful polarization of both
substrates, perfusion into the isolated rat heart
enabled direct visualization of the interconversion
between the ketone bodies along with observation of
their downstream metabolism in the Krebs cycle.
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