Abstract #1749
Pyruvate to lactate metabolism with age in normal mice measured by hyperpolarized 13C
Yiran Chen 1 , Robert Bok 1 , Subramanian Sukumar 1 , Xin Mu 1 , Ann Sheldon 2,3 , A James Barkovich 1 , Donna M Ferriero 2,3 , and Duan Xu 1,4
1
Department of Radiology and Biomedical
Imaging, University of California San Francisco, San
Francisco, CA, United States,
2
Department
of Neurology, University of California San Francisco,
CA, United States,
3
Department
of Pediatrics, University of California San Francisco,
CA, United States,
4
Joint
UCSF/UC Berkeley Graduate Group in Bioengineering, CA,
United States
In this study, we applied dynamic nuclear polarization (DNP)
technique to investigate C1 labeled 13C pyruvate to
lactate conversion across maturation in mice in vivo.
Normal mice were scanned starting on postnatal day 18
and repeated every 10 days. Experiments were conducted
on a 14.1T NMR spectrometer with real-time metabolic
imaging simultaneously with injection of hyperpolarized
pyruvate. We found that lactate level was significantly
higher at younger ages, and stabilized around P30. The
level of lactate was also independent to the
polarization or pyruvate level.
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