Abstract #0989
Hyperpolarized [1-13C]acetate kinetics and metabolism in translational animal model: cardiac real-time detection of metabolic flux of [13C]acetyl-carnitine in pigs
Alessandra Flori 1 , Matteo Liserani 2 , Francesca Frijia 3 , Vincenzo Lionetti 1 , Giulio Giovannetti 4,5 , Giacomo Bianchi 6 , Anar Dushpanova 1 , Jan Henrik Ardenkjaer-Larsen 7,8 , Giovanni Donato Aquaro 3 , Vincenzo Positano 9 , Maria Filomena Santarelli 4,5 , Luigi Landini 9,10 , Massimo Lombardi 3 , and Luca Menichetti 3,4
1
Scuola Superiore Sant'Anna, Institute of
Life Sciences, Pisa, Italy,
2
Department
of Physics, University of Pisa, Pisa, Italy,
3
Fondazione
CNR/Regione Toscana G. Monasterio, Pisa, Italy,
4
Institute
of Clinical Physiology, National Council of Research,
Pisa, Italy,
5
MRI
Unit, Fondazione CNR/Regione Toscana G. Monasterio,
Pisa, Italy,
6
Cardiac
Surgery Department, Ospedale del Cuore "G. Pasquinucci",
Fondazione CNR/Regione Toscana G. Monasterio, Massa,
Italy,
7
GE
Healthcare, Denmark,
8
Department
of Electrical Engineering, Technical University of
Denmark, Denmark,
9
MRI
Lab, Fondazione CNR/Regione Toscana G. Monasterio, Pisa,
Italy,
10
Department
of Information Engineering, University of Pisa, Pisa,
Italy
We present an analysis based on the ratio of total areas
under the curve (AUC), for real-time detection of
metabolic flux and enzymatic reactions using
[1-13C]acetate dissolution-DNP and MRS. Hyperpolarized
sodium [1-13C]acetate (150 mM) was administered in pigs
at rest and during inotropic stress with dobutamine:
[1-13C]acetate and [1-13C]acetyl-carnitine were detected
in a selected heart slice. [1-13C]acetate kinetics
displayed a typical biphasic shape and the ratio of
[1-13C]Acetyl-carnitine/[1-13C]acetate AUC showed a good
correlation with Rate Pressure Product. We proved the
feasibility of cardiac metabolic studies with
hyperpolarized [1-13C]acetate using an approach
alternative to kinetic model-based analysis, relevant
for clinical translation.
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