Hyeon-Man Baek1,
Yun-Ju Lee1, Eun-Hee Kim1, Chaejoon Cheong1,
Seung-Ho Yang2
1Division
of Magnetic Resonance, Korea Basic Science Institute, Ochang, Chungbuk,
Korea; 2Neurosurgery St. Vincent's Hospital, Catholic University,
Suwon, Gyeonggi-do, Korea
We present here that 900MHz 2D-COSY NMR can be used to obtain quantitative information of 2HG and to detect metabolic alterations in gliomas with IDH1/2 gene mutation. The measured 2HG levels in this work ranged from 0.12 to 5.64 (mean &[plusmn] SD, 2.38 &[plusmn] 1.63) &[mu] mol/g, which is consistent with our 1D 1H-NMR value (e.g., 0.29 &[plusmn] 5.79 &[mu] mol/g). The present study demonstrates the feasibility of multi-dimensional NMR technique in the detection and quantification of 2HG as a novel biomarker of IDH1/2 mutation status in glioma. In addition, our analysis revealed that a significant decrease in the concentrations of Glu, PC and Tau, and a significant increase of GPC, were observed in the IDH1/2 mutated tumors (P < 0.05).