Alessandra Laufs1,
Roshan Livingstone2, Maria Fritsch3, Julia Szendroedi4,
Juergen Bunke5, Michael Roden1, 6, Jong-Hee
Hwang1
1Institute
for Clinical Diabetology, German Diabetes Center, Dsseldorf, Germany; 2Department
of Radiology, Christian Medical College, Vellore, India; 3Medical
University of Vienna, Vienna, Austria; 4Institute for Energy
Metabolism, German Diabetes Center, Dsseldorf, Germany; 5Philips
Healthcare, Hamburg, Germany; 6Department of Metabolic Deseases,
University Clinics Dsseldorf, Dsseldorf, Germany
Changes in phosphoenolpyruvate (PEP) concentrations of the liver were quantified before and after a high-carbohydrate meal using 31P MRS in six healthy subjects at 3T. Quantification of PEP was performed using matching phantoms and an external reference in order to correct for excitation pulse profile, coil loading, B1 field inhomogeneity, T1 relaxation time (T1 = 0.82 0.16 s, separately assessed, N = 5)and the amount of liver fat. The concentration of PEP 140 min after the meal was significantly decreased from 1.14 0.22 mmol/l to 0.86 0.22 mmol/l (N = 6, p = 0.01, paired t-test).