Sonal Josan1,
2, Ralph Hurd3, Yi-Fen Yen2, Adolf Pfefferbaum1,
4, Daniel Spielman2, Dirk Mayer1, 2
1SRI
International, Menlo Park, CA, United States; 2Radiology, Stanford
University, Stanford, CA, United States; 3GE Healthcare; 4Psychiatry
and Behavioral Sciences, Stanford University, Stanford, CA, United States
Spectroscopic imaging with hyperpolarized 13C substrates has been widely used to measure metabolic processes in real time in vivo. There has been growing interest recently in using hyperpolarized [2-13C]pyruvate, to follow the 13C label into metabolic products including glutamate (in exchange with α-ketoglutarate in the TCA cycle), citrate, acetyl-carnitine and acetoacetate as well as lactate and alanine. CSI with [2-13C]Pyr is challenging given the wide spectral dispersion of the resonances. This work uses a spectrally selective excitation to perform 3D CSI of spectral sub-bands containing metabolites of interest. Dynamic data is acquired from multiple sub-bands alternately in vivo in rat heart.