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Abstract #3922

Alternating Acquisition for Quantification of Pyruvate Metabolism in Hyperpolarized 13C Studies

Seungwook Yang1, Joonsung Lee1, Yoonho Nam1, Eunhae Joe1, Jae-Eun Suk2, Ho-Taek Song2, Dong-Hyun Kim1

1Electrical and Electronic Engineering, Yonsei University, Seoul, Korea; 2Department of Radiology, College of Medicine, Yonsei University, Seoul, Korea


A method for accurate quantification of hyperpolarized pyruvate and the product lactate in in vitro experiment is described. By using a narrow-band RF excitation pulse with alternating acquisition at every TR, metabolic product can be selectively excited with a higher flip angle for increased SNR while the hyperpolarized magnetization of the substrate can be minimally perturbed with a low flip angle. Also, baseline signals from neighboring resonances can be suppressed to accurately quantify the metabolism kinetics. Furthermore, with the modified version of the two-site exchange model that is also presented, calculation of the rate constants associated with the conversion is also possible.