Abstract #3914

Metabolic Profiling of Experimental Tumors Using Hyperpolarized [1-13C] Pyruvate: Evaluating Cell Type Specificity and Effects of Tumor Environment.

Georgios Batsios¹, Divya Vats¹, Catherine Germanier¹, Michael Batel², Marcin Krajewski¹, Markus Rudin¹,³

¹Institute for Biomedical Engineering, ETH and University of Zurich, Zurich, Switzerland; ²Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland; ³Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland

Hyperpolarized ¹³C magnetic resonance spectroscopy and imaging is a rapidly expanding field with many applications, particularly in the field of cancer. In this study we compare the lactate dehydrogenase (LDH) activity by measuring the conversion of pyruvate to lactate using same type of tumor cells growing at different implantation sites and different genetic background mice and using different tumor cell lines. We show that the pyruvate to lactate conversion depends mostly on the tumor type and not on the tumor environment. This indicates that the pyruvate processing through the LDH reaction reflects an intrinsic property of the tumor cell line.