Naira P. Martinez Vera1,
Klaus Langer2, Iavor Zlatev2, Robert Wronski3,
Manfred Windisch3, Ewald Auer3, Hagen von Briesen4,
Sylvia Wagner4, Motti Deutsch5, Claus Pietrzik6,
Franz Fazekas1, Reinhold Schmidt1, Stefan Ropele1
1Department
of Neurology, Medical University of Graz, Graz, Styria, Austria; 2Institut
fr Pharmazeutische Technologie und Biopharmazie, Mnster, North
Rhine-Westphalia, Germany; 3JSW Life Sciences GmbH, Grambach,
Styria, Austria; 4Department of Cell Biology & Applied Virology,
Fraunhofer-Institute for Biomedical Engineering, St. Ingbert, Saarland,
Germany; 5Physics Department, , Schottenstein Center for the
Research and Technology of the Cellome, Bar Ilan University, Ramat Gan,
Israel; 6Institute of Pathobiochemistry,, University Medical
Center of the Johannes Gutenberg-University Mainz, Mainz,
Rhineland-Palatinate, Germany
Nanoparticles (NP) have been suggested as a vehicle to selectively transport drugs over the blood brain barrier. In this work we investigated the feasibility of measuring the accumulation of HSA based NP in the rat brain after intravenous injection. The NP were labeled with magnetite in order to achieve a significant T1 relaxivity effect. The measurement of NP concentration was based on a histogram fitting technique which allowed to globally assess T1 changes in gray and white matter. This approach proved to be very sensitive and was validated with results from autofluorescence imaging.