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Abstract #3421

in vivo 2D L-COSY Detects Decreased PC in Gliomas After Treatment with MN58b, a Choline Kinase Inhibitor

Gaurav Verma1, Manoj Kumar1, Sona Saksena2, Ranjit Ittyerah2, Anatoliy V. Popov2, Jenny Li3, M. Albert Thomas3, Edward James Delikatny2, Harish Poptani1

1Department of Radiology and Radiation Oncology, University of Pennsylvania, Philadelphia, PA, United States; 2Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States; 3Department of Radiological Sciences, University of California Los Angeles, Los Angeles, CA, United States


The treatment response of rat gliomas to a choline-kinase inhibitor, MN58b, was quantified using a 2D localized correlated spectroscopy (L-COSY) sequence in a 9.4T horizontal bore scanner. In vivo 2D spectra from six normal brains, five untreated tumors and three treated tumors, showed uniquely resolvable peaks of several metabolites including phosphocholine (PC) and glycerophosphocholine (GPC). The PC/GPC ratio was found to be elevated in tumor compared to normal rat brain (1.04 vs. 0.88), and then found to decrease to an average of 0.86 following treatment with MN58b.