Characterization of an Orthotopic Mouse Model Developed from Human Glioblastoma Spheres

Feriel Tiar¹, Teodora-Adriana Perles-Barbacaru¹, Michelle El-atifi¹, Didier Wion¹, Laurent Pelletier¹, Hana Lahrech¹, Francois Berger¹

A new orthotopic mouse model derived from human glioblastoma spheres was recently developed. Tumor growth kinetics and response to temozolomide treatment was assessed by MRI. Similar to clinically encountered glioblastomas, this model develops over several months, has an infiltrating growth pattern, mimics their cellular heterogeneity and presents a temozolomide failure. It will therefore be useful for the preclinical evaluation of new treatment strategies.