Effects of Different Anesthesia on the Resting-State Networks in Rodents

Johnny Ng¹, Jessica Roman¹, Edmund Wong¹, David Carpenter¹, Cheuk Tang¹, ²

Functional imaging in animal models is difficult to perform, especially in awake experiments. Resting-state fMRI is the most convenient way to assess brain function with the animal under anesthesia. The aim of this study is to investigate the effects of three commonly used anesthetic isoflurane, medetomidine and ketamine in rodent experiments and their effects on brain function. 14 mice were used in this study. MELODIC ICA and dual-regression analysis were implemented. Increased coactivation has been found in several networks with medetomidine compared with ketamine or isoflurane alone.