Rheal A. Towner1,
Nataliya Smith1, Philippe Garteiser1, Debra Saunders1,
Florea Lupu2, Robert Silasi-Mansat2, Dario C. Ramirez3,
Sandra E. Gomez-Mejiba3, Ronald P. Mason4, Marilyn I.
Ehrenshaft4, Fernando Bozza5, Marcus Frenandes de
Oliveira6, Hugo C. Castro Faria-Neto7
1Advanced
Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma
City, OK, United States; 2Cardiovascular Biology, Oklahoma Medical
Research Foundation, Oklahoma City, OK, United States; 3Laboratory
of Experimental and Therapeutic Medicine, National University of San Luis,
San Luis, Argentina; 4Laboratory of Pharmacology & Chemistry,
National Institute of Environmental Health Sciences, Research Triangle Park,
NC, United States; 5Instituto de Pesquisa Clinica Evandro Chagas,
Fundao Oswaldo Cruz, Rio de Janeiro, RJ, Brazil; 6Instituto de
Bioqumica Mdica, Universidade Federal do Rio de Janeiro, Rio de Janeiro,
RJ, Brazil; 7Instituto de Pesquisa Clinica Evandro Chagas,
Fundao Oswaldo Cruz, Rio de Janeiro, Brazil
Oxidative stress from free radicals plays a major role in sepsis. A combination of immuno-spin-trapping (IST) and targeted molecular magnetic resonance imaging (mMRI) was used to detect in vivo levels of membrane-bound radicals (MBR) in brain tissues from mice with cecal ligation puncture (CLP)-induced sepsis. The spin trapping compound DMPO (5,5-dimethyl pyrroline N-oxide) was used to trap radicals. An anti-DMPO probe (anti-DMPO antibody covalently bound to an albumin-Gd-DTPA-biotin construct was used to detect MBR in vivo with mMRI. This method can be applied towards any radical-associated pathological condition for the in vivo assessment of membrane-bound protein and/or lipid radical levels.