Aditya N. Bade1,
Biyun Zhou2, Santhi Gorantla1, Jiangtao Luo3,
Howard E. Gendelman1, Michael Douglas Boska4, Yutong
Liu4
1Pharmacology
and Experimental Neuroscience, University of Nebraska Medical Center, Omaha.,
Omaha, NE, United States; 2Anesthesiology Department, Tongji
Medical College, Huanzhong University of Science and Technology, Wuhan, Hubei,
China; 3Department of Biostatistics, University of Nebraska
Medical Center, Omaha., Omaha, NE, United States; 4Department of
Radiology, University of Nebraska Medical Center, Omaha., Omaha, NE, United
States
Mn2+ enhanced MRI (MEMRI) can be used to detect a number of neuropathological events. However, the mechanisms for Mn2+ signal enhancement in inflammatory pathobiological states remains controversial. Here, we applied intracranial LPS injection to create an inflammatory lesion and quantified signal enhancement after Mn2+ treatment. Combined with our previous in-vitro study of Mn2+ uptake in activated glial cells and neurons, we conclude that activated glia do not directly induce signal enhancement in MEMRI, but signal enhancement in MEMRI results from the increased neuronal activity induced by gliosis, mainly astrogliosis, stimulating neuronal Mn2+ uptake.