Metabolic Profiling of RG2, F98 and C6 Glioma Models Using 1H-MRS and Ex-Vivo 1H HRMAS MRS

Nicolas Coquery¹, ², Vasile Stupar, ²³, Rgine Farion, ²³, Sverine Maunoir-Regimbal⁴, Emmanuel Luc Barbier, ²⁵, Chantal Rmy¹, ², Florence Fauvelle⁴

Magnetic Resonance-based spectroscopy (MRS) is a powerful method to investigate the metabolic consequences of cancer disease. In vivo MRS provides information regarding tumor growth and response to treatment. These information can be refined with ex vivo High Resolution Magic Angle Spinning (HRMAS) MRS. With these two approaches a huge amount of information can thus be gathered that might render the analysis difficult in clinic. We propose here to use statistical tools such as PLS-DA to discriminate tumoral tissue from normal tissue. PLS-DA analysis is also able to show a clear separation between three glioma models in rat and to highlight the metabolites that contribute to this separation despite inter-individual variability.