Abstract #0808

Mutation in Isocitrate Dehydrogenase 1 (IDH1) Leads to Increased T2, ADC and Decreased Lactate and Glutamate in Glioblastoma Model

Jelena Lazovic¹, Soto Horacio², Sichen Li³, Albert Lai³, Linda Liau², Robert Prins², Timothy F. Cloughesy⁴, Whitney Pope⁵

¹Radiology, University of California at Los Angeles, Los Angeles, CA, United States; ²Neurosurgery, University of California, Los Angeles, CA, United States; ³Neuro-Oncology, University of California, Los Angeles, CA, United States; ⁴Neuro-Oncology, UCLA, Los Angeles, CA, United States; ⁵Radiology, University of California, Los Angeles, CA, United States

Recent reports strongly correlate presence of isocitrate dehydrogenase 1 (IDH1) mutation (IDH1-R132) with improved overall survival among glioblastoma patients. This mutation is associated with production of 2-hydroxyglutaric (2-HG) acid, with not well established role in malignant progression. Human glioblastoma cell line (U87) was modified to overexpress mutated isocitrate dehydrogenase 1 (IDH1) R132 in order to determine if 2-hydroxyglutaric acid could be detected in vivo and the consequence on tumor growth and metabolism. We found glioblastoma that overexpressed IDH1-R132 to have significantly increased growth rate along with accumulation of 2-HG and reduction in lactate and glutamate.