Abstract #0658

In Vivo Assessment of IDH Status in Glioblastoma Using 2D 13C Dynamic CSI of Hyperpolarized &[alpha]-Ketoglutarate

Myriam Marianne Chaumeil¹, Peder E.Z. Larson¹, Hikari A. I. Yoshihara¹, Daniel B. Vigneron¹, Sarah J. Nelson¹, Russell O. Pieper², Joanna J. Phillips², Sabrina M. Ronen¹

¹Radiology, University of California, San Francisco, San Francisco, CA, United States; ²Neurological Surgery, University of California, San Francisco, San, CA, United States

We previously showed that hyperpolarized &[alpha]-KG was a promising probe to inform on isocitrate dehydrogenase (IDH1) mutational status in cell lysates and live perfused cells. In this study, we translated our methods in vivo and evaluated the potential of hyperpolarized &[alpha]-KG to assess IDH status in orthotopic GBM tumors at clinical field strength. Following injection of hyperpolarized &[alpha]-KG, hyperpolarized 2-hydroxyglutarate formation was detected in IDH-mutant tumors, but not in in IDH wild-type, using 2D ¹³C dynamic CSI. This initial study demonstrates that HP &[alpha]-KG can inform on IDH mutational status in vivo through the dynamic assessment of its metabolism.