Microscopic 3D-DTI of Tumor Cell Migration, Numerical Modeling and Two- Photon Microscopic Imaging

Ulysse Gimenez¹, Florence Appaix¹, Teodora-Adriana Perles-Barbacaru¹, Franck Mauconduit¹, Marie-France Nissou¹, Emilie Langard¹, Laurent Pelletier¹, Francois Berger¹, Didier Wion¹, Boudewijn van der Sanden¹, Hana Lahrech¹

A microscopic 3D-DTI is applied on a mouse glioma model using GFP transfected Glio6 cells to detect tumor cell migration. Two-photon microscopy is used for validation. Monte-Carlo simulations of water diffusion in numerical models of cerebral tissue geometry with micro-architecture changes are developed. FA decrease is observed in the corpus callosum where tumor cell invasion is particularly detected by microscopy. Typical elongated tumor cells indicate migration along the fibers. FA changes simulations versus extra / intracellular and white-matter / grey-matter show similar tendency as detected experimentally. The Glio6 model and 3D-DTI constitute a powerful tool to study tumor cell migration.