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Abstract #4432

HRMAS NMR Spectroscopic Analysis and Quantification of Marrow Adiposity in the B6 Mouse Model After Exposure to Rosiglitazone Treatment

Keerthi Shet1, Hikari Yoshihara1, John Kurhanewicz1, Ann Schwartz2, Cliff J. Rosen3, Xiaojuan Li1

1Radiology, University of California, San Francisco, San Francisco, CA, United States; 2Epidemiology and Biostatistics, University of California, San Francisco, San Francisco, United States; 3Jackson Laboratory, Bar Harbor, ME


Clinical 1H-MRS studies have shown that increased bone-marrow adiposity is associated with decreased BMD in patients with osteoporosis/osteopenia. Marrow adipose tissue(MAT) from the C57BL/6J(B6) mice was analyzed using HRMAS-NMR following exposure to type-2-diabetes-drug�rosiglitazone. We observed significant changes in MAT composition along with the rosiglitazone-induced increase in adiposity. In particular, the decreased unsaturated-lipids and increased saturated-lipids may be correlated with decreased bone strength with rosiglitazone treatment. Thus, consistent with recent observation in-vivo that decreased MAT unsaturation level is associated with fracture-risk, the fatty-acid unsaturation/saturation level of MAT can be a potential imaging marker for bone quality and fracture.