Abstract #0270

Non-Invasive Assessment of IDH Status in Glioblastoma Using Dynamic 13C MRS of Hyperpolarized α-Ketoglutarate

Myriam Marianne Chaumeil¹, Sarah Woods¹, Robert M. Danforth¹, Hikari Yoshihara¹, Alessia Lodi¹, Aaron Robinson², Joanna J. Philips²,³, Sabrina M. Ronen¹

¹Radiology, University of California, San Francisco, San Francisco, CA, United States; ²Neurological Surgery, University of California, San Francisco, San Francisco, CA, United States; ³Pathology, University of California, San Francisco, San Francisco, CA, United States

The mutational status of isocitrate dehydrogenase (IDH1) was assessed using dynamic ¹³C MRS of hyperpolarized (HP) α-ketoglutarate in two glioblastoma cells lines: U87 transduced with IDH1 wild-type (U87IDHwt) or IDH mutant (U87IDHmut). Following injection of HP α-KG, HP 2-hydroxyglutarate and HP glutamate were detected in U87IDHmut lysates. Only HP glutamate was visible in U87IDHwt lysates. Injection of HP α-KG in live perfused U87IDHwt cells resulted in dynamic HP glutamate build-up whereas no HP glutamate was detected in perfused U87IDHmut cells. This pioneer study demonstrates that HP α-KG can inform on IDH mutational status through the dynamic assessment of its metabolism.