Neurochemical Profile of the Striatum & Hippocampus in Mice at 16.4T using In Vivo 1H NMR Spectroscopy

Dinesh K. Deelchand¹, Isabelle Iltis¹, Gregor Adriany¹, Emily Colonna¹, Malgorzata Marjanska¹, Kamil Ugurbil¹, Pierre-Gilles Henry¹

In this work, we demonstrate that highly resolved ¹H NMR spectra can be obtained in different regions of the mouse brain in vivo at 16.4 T. Spectra were acquired in the striatum and hippocampus of anesthetized mice using the LASER sequence (TE of 16.5 ms) with a voxel size less than 5 l. Using LCModel analysis, 16 and 17 metabolites (out of 19) were quantified in the hippocampus and striatum respectively with Cramr-Rao Lower bounds < 20%. Future studies in mouse models will benefit from the excellent spectral resolution and accurate localization obtained at 16.4 T.