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Abstract #3443

Novel Approach for the Assessment of the Bioavailability of Exogenous Phosphate by In Vivo Dynamic 17O & 31P MRS & MRI

Gheorghe D. Mateescu1,2, Chris A. Flask1,3, Jeffrey L. Duerk1,3

1Radiology, Case Western Reserve University, Cleveland, OH, United States; 2Chemistry, Case Western Reserve University, Cleveland, OH, United States; 3Biomedical Engineering, Case Western Reserve University, Cleveland, OH, United States


This communication presents a novel application of in vivo dynamic 17O and 31P MRS and MRI for the assessment of the bioavailability of exogenous phosphate (dietary or pharmaceutical). The method consists of administration of 17O-enriched phosphate to mice, followed by dynamic measurement of the 17O label transfer to body water, that results from the hydrolytic reactions of phosphate metabolism. Alternately, an efficient measurement could be done in 31P MR with 17O decoupling. This new approach may become important in relating phosphate homeostasis defects to metabolic or other diseases.