Abstract #2774

Identification of Endogenous Proteins Correlated with Amide Proton Transfer (APT) Imaging Contrast using Proteomic Analysis

Kun Yan¹, Zongming Fu², Jennifer Van Eyk³, Silun Wang¹, Jinyuan Zhou¹

¹Radiology, Johns Hopkins University, Baltimore, MD, United States; ²Pediatrics, Johns Hopkins University, Baltimore, MD, United States; ³Bayview Proteomics Center, Johns Hopkins University, Baltimore, MD, United States

Amide proton transfer (APT) imaging as a specific type of chemical exchangeCdependent saturation transfer (CEST) MRI technique can distinguish brain tumor from normal tissue. To determine which protein(s) contribute to the APT signal, protein profiles of rat 9L tumor and normal brain tissues were compared by proteomic studies. Six significantly up-regulated cytosolic proteins were identified and supposed to correlate with APT hyper-intensity in tumor.