Verification of Metabolite Peak Change During Chondrogenesis of Human Mesenchymal Stem Cells using Proton NMR

Moo-Young Jang¹, So-Hee Park¹, Jung-Woog Shin^1,2, Chi-Woong Mun^1,2

This paper is intended to investigate on metabolite changes of chondrogenic hMSCs using 1H NMR to confirm the differentiation. Chondrogenic hMSCs were resuspended to 0.9% saline D2O and was filled in 5mm NMR tube (2x106cells/500l). We confirmed differentiation by alcian blue staining. NMR spectrum data were acquired using 500MHz NMR spectrometer with the following parameters: PRESAT sequence, relaxation delay=20ms, 45 pulse, NT=512. We confirmed that peak intensities of 1H MR spectrum tend to increase at 1.33/1.48/1.49/2.94-2.97/3.23/3.56/ 3.78 ppm after the onset of chondrogenic differentiation from hMSCs. On the other hand, lipid and fatty acid peaks were reduced during the differentiation.