Abstract #1440

Precision Evolution of the Neuroglial Metabolic Fluxes with the Experimental Conditions, When using Two-Compartment Modeling Applied to [2-13C] Acetate Dynamic MRS Studies

Bernard Lanz¹, Lijing Xin¹, Rolf Gruetter¹,²

¹Laboratory for Functional & Metabolic Imaging, Ecole Polytechnique Fdrale de Lausanne, Lausanne, Switzerland; ²Department of Radiology, Universities of Lausanne & Geneva, Lausanne & Geneva, Switzerland

[2-¹³C]acetate infusion coupled with ¹³C MRS enables the separate assessment of glial and neuronal Krebs cycle fluxes and the determination of the apparent neurotransmission flux with higher accuracy than ¹³C labeled glucose, due to the glial specificity of acetate metabolism. Furthermore, these fluxes are determined with different precisions, which might strongly depend on the experimental conditions. In this study, we analyze the effect of the experiment duration, the time resolution and the noise level of the data on the standard deviation of the neuroglial fluxes, determined with Monte-Carlo simulation.