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Abstract #1088

The Effects of Platelet-Derived Growth Factor on Vascular Permeability Studied by MRI

Yann Jamin1, Jessica K. R. Boult1, Lauren C. Baker1, Simon Walker-Samuel2, Arne stman3, Simon P. Robinson1

1CRUK & EPSRC Cancer Imaging Centre, The Institute of Cancer Research & Royal Marsden NHS Trust, Sutton, United Kingdom; 2UCL Centre for Advanced Biomedical Imaging, Department of Medicine & Institute of Child Health, University College London, United Kingdom; 3Cancer Center Karolinska, Karolinska Institutet, Stockholm, Sweden


Mature, more patent vasculature is predicted to have more complete endothelial lining, basal membrane and smooth muscle and hence reduced permeability. The latter has not been yet unequivocally demonstrated in vivo. We have used DCE-MRI to interrogate tumours derived from B16 melanoma cells engineered to express PDGF-BB (B16/PDGF), which have previously been shown to exhibit a significantly more mature vasculature, compared to wild-type B16 melanomas (B16/ctr). Results show that PDGF/B16 tumours present a lower IAUCG60 and T1 than B16/ctr tumours, suggesting that B16/PDGF tumours present a reduced vascular permeability compared to B16/ctr tumours.