In Vivo Detection & Quantification of Diet Induced Changes in Adipose Tissue Composition by Non Linear NMR Spectroscopy

Rosa Tamara Branca¹, Warren Sloan Warren²

In vivo analysis of adipose tissue composition by NMR often requires water suppression, accurate voxel localization to avoid tissue interfaces, and the use of relatively small voxels and tedious localized shimming procedures to minimize local magnetic field inhomogeneities. In this report we show how non linear NMR spectroscopy can be used to acquire high resolution spectra of lipid depots spread over large (several cubic centimeter) volumes, without using localized shimming procedures or water suppression modules. We show how we calibrate and validate this method for samples containing known amounts of fatty acids, and how we use it in vivo to measure the total adipose tissue composition change induced by diet intervention in mice.