Abstract #0657

Simultaneous Investigation of Cardiac Pyruvate Dehydrogenase Flux, Krebs Cycle Metabolism & Intracellular Ph using Hyperpolarized [1,2-13C2] Pyruvate

Albert P. Chen¹, Ralph E. Hurd², Marie A. Schroeder^3,4, Angus Z. Lau^4,5, Yi-Ping Gu⁴, Wilfred W. Lam⁴, Jennifer Barry⁴, James Tropp⁶, Charles H. Cunningham^4,5

¹GE Healthcare, Toronto, ON, Canada; ²GE Healthcare, Menlo Park, CA, USA; ³Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, United Kingdom; ⁴Imaging Research, Sunnybrook Health Sciences Centre, Toronto, ON, Canada; ⁵Deptartment of Medical Biophysics, University of Toronto, Toronto, ON, Canada; ⁶GE Healthcare, Fremont, CA, USA

Utilization of either C1 or C2 labeled pre-polarized pyruvate as a tracer can only afford a partial view of cardiac pyruvate metabolism. If pyruvate was labeled at both C1 and C2 positions, then it would be possible to observe the down stream metabolites that were the results of both PDH and Krebs cycle flux with a single tracer bolus. Intracellular pH could also be estimated from the same data, provided the ¹³CO₂ signal has adequate SNR. This study demonstrated the feasibility of simultaneous investigation of cardiac PDH flux, Krebs cycle metabolism and intracellular pH in vivo, by using hyperpolarized [1,2-¹³C₂]pyruvate.