Metabolism of Hyperpolarized U-13C-d7-D-Glucose in Living Breast Cancer Cell Cultures

Talia Harris¹, Lucio Frydman¹, Hadassa Degani²

Elevated glycolytic rates are a known feature of many cancers. The recent development of a method to hyperpolarize nuclear spins more than 10,000x in the liquid states opens up new opportunities to follow metabolic processes non-invasively with high temporal resolution using 13C NMR. In this study we utilize a perfusion-injection system to study the metabolism of hyperpolarized U-13C-d7-D-Glucose in living T47D breast cancer cells. The conversion of Glucose Lactate as well as labeling of other metabolites is seen. Further, the conversion of GlucoseLactate by T47D cells is shown to have the same concentration dependence as the uptake of radioactively labeled 2-deoxy-D-Glucose.