Abstract #4824

A Comparative Study of Frequency and Time Domain Data Analysis of HR-MAS 1H NMR Data from ApcMin/+ Mouse Gut Tumours

Basetti Madhu¹, Santiago Uribe Lewis², Adele Murrell², John R. Griffiths¹

¹Molecular Imaging, Cancer Research UK Cambridge Research Institute, Cambridge, England, United Kingdom; ²Epigenetics Imprinting, Cancer Research UK Cambridge Research Institute, Cambridge, England, United Kingdom

In this study we compared HR-MAS 1H NMR spectroscopy data of mouse gut tumour tissue biopsies obtained by time domain (ER-QUEST) and frequency domain (LCModel) analysis methods., Our results show that LCModel fitting resulted in either equal or smaller fractional uncertainties in the estimates of metabolites in comparison to the ER-QUEST method. The LCModel method also showed greater ease and robustness in the fitting of HR-MAS ¹H NMR data from the experimental tumours.