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Abstract #4801

Histological Validation of the Cerebral Blood Volume Quantification in a C6 Brain Tumor Model Using RSST1-MRI with an Intravascular Contrast Agent: Gd-ACX

Adriana-Teodora Perles-Barbacaru1,2, Boudewijn Van der Sanden3, Rgine Farion1, Christoph Segebarth1, Hana Lahrech1

1Functional and Metabolic Neuroimaging, Grenoble Institute of Neuroscience, Grenoble, France; 2Caltech Brain Imaging Center, Beckman Institute, Pasadena, CA, United States; 3Rayonnement Synchrotron et Recherche Mdicale, Grenoble Institute of Neuroscience, Grenoble, France


The cerebral blood volume fraction (CBVf) quantification by MRI remains complex in the tumor due to the contrast agent (CA) leakage through the blood brain barrier. Gd-ACX (α-cyclodextrin complexed to gadolinium), a novel CA was shown to remain in the vascular space in a C6 brain tumor model and was used for CBVf mapping in microvasculature permeable for Gd-DOTA. Here, the use of Gd-ACX for tumor CBVf quantification was validated using histological vascular morphometry. After selecting the tumor vessels perfused by the Hoechst dye, we found a quantitative equivalence for the CBVf obtained by MRI and by vascular morphometry.