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Abstract #4176

Cellular MRI Assessment of Magnetic Fluorescent Bead Labeled Macrophage Accumulation Following High Intensity Focused Ultrasound (HIFU) Induced Damage in a Murine Model

Hilary Hancock1, Eric M. Gold1, Bobbi K. Lewis1, Melissa Smith1, Victor Frenkel1, Joseph A. Frank1,2

1Radiology and Imaging Sciences, NIH, Bethesda, MD, United States; 2National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD, United States


This study investigated in vivo labeling of monocytes with SPIO/fluorescent 40nm beads followed by Cellular MRI and fluorescent microscopy to determine the effects of ablative or pulsed high intensity focused ultrasound (HIFU) in a murine model. Pulsed HIFU exposures exhibited smaller regions of edema and hypointense regions, confined to superficial muscle and dermis, on T2*W images with smaller amounts of immune response within tissues compared to ablated tissues.