Ming-Hung Chen1, Gin-Chung Liu2,3,
Twei-Shiun Jaw2,4, Yu-Ting Kuo2,3, Chiao-Yun Chen2,3,
Yun-Ming Wang1
1Department of Biological Science and
Technology, National Chiao Tung University, Hsinchu, Taiwan; 2Department
of Medical Imaging, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;
3Department of Radiology,Faculty of Medicine,College of Medicine,
Kaohsiung Medical University, Kaohsiung, Taiwan; 4Department of
Radiology,Faculty of Medicine,College of Medicine, Kaohsiung Medical University, Kaohsiung,
Taiwan
In
this study, the various core sizes of manganese ferrite nanoparticles
(MnFe2O4) conjugated with D4 peptide (MnFe2O4-PEG-D4) were synthesized. The
high relaxivity MnFe2O4 nanoparticles were obtained by thermal decomposition
of Iron acetylacetonate and manganese acetylacetonate in hydrophobic solution
at high boiling process. The surface of MnFe2O4 nanoparticles were coated
with polyethylene glycol (PEG) and EGFR peptide ligand (D4:
Leu-Ala-Arg-Leu-Leu-Thr) to improve their dispersion and ability to target
EGFR. The negative signal enhancement of EGFR expressing cancer cells (SKBR-3
and PC-3) were significantly higher than that of low EGFR expressing cells
(HEK-293).