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Abstract #3548

Repeatability of ASL Cerebral Blood Flow and BOLD Cerebrovascular Reactivity Measurements Using a Computer-Controlled Gas Delivery System in a Pediatric Animal Model

Jeff D. Winter1, Jorn Fiestra2,3, Stephanie Dorner4, Joseph A. Fisher5,6, Keith St. Lawrence7,8, Andrea Kassner1,9

1Physiology and Experimental Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada; 2Department of Medical Imaging, University Health Network, Toronto, Ontario, Canada; 3Department of Neurosurgery, University Health Network, Toronto, Ontario, Canada; 4Respiratory Therapy, University Health Network, Toronto, Ontario, Canada; 5Anaesthesiology, University Health Network, Toronto, Ontario, Canada; 6Physiology, University of Toronto, Toronto, Ontario, Canada; 7Imaging Division, Lawson Health Research Institute, London, Ontario, Canada; 8Medical Biophysics, University of Western Ontario, London, Ontario, Canada; 9Medical Imaging, University of Toronto, Toronto, Ontario, Canada


Cerebrovascular reactivity (CVR) caused by a CO2 stimulus may improve the assessment of childhood cerebrovascular disease. Reliable BOLD-based CVR measures may be obtained using precise model-driven prospective end-tidal CO2 targeting (MPET). In this study, we adapted the method of MPET of CO2 to anesthetised and ventilated animals. BOLD CVR repeatability was assessed in nine mechanically ventilated juvenile pigs. We found excellent BOLD-CVR repeatability (intra-class correlation coefficient > 0.84), which was similar to baseline ASL cerebral blood flow repeatability. Translation of this method to pediatric imaging will enable CVR imaging in small children who require anesthetic for imaging procedures.