Simultaneous Quantification of Glycine- And Taurine-Conjugated Bile Acids, Total Bile Acids and Phospholipids in Human Bile by Using 1H MRS

Omkar B. Ijare¹, Tedros Bezabeh¹, Nils Albiin², Annika Bergquist², Urban Arnelo², Bo Lindberg², Ian C.P. Smith¹

Bile acids, phospholipids and cholesterol are major lipid components in bile. We propose a robust method for the simultaneous quantification of glycine-conjugated bile acids (GCBAs), taurine-conjugated bile acids (TCBAs), total bile acids (TBAs) and phospholipids (PLs). GCBAs and TCBAs have been quantified using peak areas of their characteristic methylene signals resonating at 3.73 and 3.07 ppm, whereas TBA and PLs were quantified using their methyl and trimethylammonium signals resonating at 0.65 and 3.22 ppm respectively. The peak areas of these lipid signals were obtained simultaneously by deconvolution, making the method robust. This method could be extended to in vivo applications.