Xing Chen1, Anke Henning1, Matteo
Pavan1, Susanne Heinzer-Schweizer1, Marco Toigo2,3,
Peter Bsiger1
1Institute for Biomedical Engineering,
University and ETH Zurich, Zurich, Switzerland; 2Institute of
Physiology and Zurich Center for Integrative Human Physiology (ZIHP),
University of Zurich, Switzerland; 3Exercise Physiology, Institute
of Human Movement Sciences, ETH Zurich, Switzerland
13C
MRS glycogen was detected by combined ISIS-localized NOE and proton decoupling
in human calf muscle, with the ERETIC signal as a synthetic reference
standard for quantification. Reproducibility tests were performed regarding
the quantified signal intensities, SNR enhancement factors and ERETIC signal
stability. In conclusion, the combination of NOE and decoupling can enhance
the glycogen signal at a reproducible level and enables higher fitting and
thus quantification reliability. As ERETIC signal stability proved to be
unaffected by SNT enhancement, it is promising to use the ERETIC signal as
reference for absolute quantification among different subjects and
experiments.