An Optimized T1nom Approach for Super Fast Measuring Enzyme Kinetics in Vivo Using Saturation Transfer Technique

Qiang Xiong¹, Fei Du¹, Xiao-hong Zhu¹, Jianyi Zhang^1,2, Wei Chen¹

A novel approach (T1nom) is presented for saturation transfer (ST) technique that is suitable for super fast measuring and quantifying enzyme kinetics in vivo. The T1nom approach features with arbitrarily repetition time, saturation time and flip angle while maintaining simple quantification algorithm that can be only achieved with very long TR and saturation time in the case of conventional ST approach. The T1nom approach was optimized by using numerical simulations. The results gave the guidelines for finding TR/flip angle pairs that can give rise to be most accurate measurements of kinetic rate constants within a given scan time. Finally, a practical procedure is given for intervention/stimulation experiments where changes of compound pool size ratios may be encountered.