Mor Mishkovsky1,2, Arnaud Comment1,2,
Rolf Gruetter1,3
1Laboratory for Functional and
Metabolic Imaging , Ecole Polytechnique Fdrale de Lausanne, Lausanne,
Switzerland; 2Department of Radiology, Universit de Lausanne,
Lausanne, Switzerland; 3Departments of Radiology, Universits de
Lausanne et Genve, Lausanne and Genve, Switzerland
In vivo localized 13C
MRS of a rat brain was performed in a 9.4T animal scanner after infusion of a
hyperpolarized 1-13C and 13C2 sodium acetate
solution. It is demonstrated that hyperpolarized 13C-labeled
acetate rapidly enters the brain and its metabolism can be detected within
the time window defined by the lifetime of the carboxyl carbon hyperpolarized
spin state. We present a new sequence designed to transfer the carboxyl
nuclear polarization onto the methyl carbon spin of 13C2 acetate in order
to assign the metabolic peaks observed in
vivo.