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Abstract #3213

in Vivo Quantification of Intervertebral Disc Collagen Content Using Magnetization Transfer Ratio Mapping

Chenyang Wang1,2, Walter Witschey3, Ari Goldberg4, Mark Elliott2, Joseph Calabro5, Ari Borthakur2, Ravinder Reddy2

1Bioengineering, University of Pennsylvania, Philadelphia, PA, United States; 2Radiology, University of Pennsylvania, Philadelphia, PA, United States; 3Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia, PA, United States; 4Radiology, Hospital of the University of Pennsylvania, Philadelphia, PA, United States; 5Siemens Medical Solutions


Magnetization transfer (MT) has previously been shown to be sensitive to collagen content in biological tissues. In this study, we quantified MT effect by computing the MT ratio of the intervertebral disc (IVD) on a pixel-by-pixel basis, from in vivo MT MR images. The resulting MT ratio map of healthy IVD showed significantly higher MT ratio in the collagen-rich annular fibrosis compared to the proteoglycan-rich nucleus pulposus. In contrast, the MT ratio of degenerated IVD nucleus pulposus appeared elevated, which suggests possible increase in collagen content in the otherwise proteoglycan-rich tissue as a result of degeneration.