Meeting Banner
Abstract #2985

Detection of Glycosaminoglycans Using Positive CEST Approach

Elena Vinogradov1, Robert E. Lenkinski1

1Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States


Chemical Exchange Saturation Contrast utilizes selective presaturation of a small pool of exchanging protons and is manifested in the decrease of the free water signal. Recently, CEST method has been applied successfully to detect glycosaminoglycans (GAG) in cartilage. CEST contrast is negative, resulting in decreased signal from areas with high agent (GAG) concentration. An alternative scheme, positive CEST (pCEST), results in the background suppression and positive contrast when signal is increased due to the presence of the exchanging agent. Here we evaluate application of the pCEST to detect GAG in solutions and ex-vivo samples.