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Abstract #2343

Simple and Efficient Image Processing Techniques to Improve the Registration Between the MR and Light Microscopy Images

Xia Li1,2, Ann Choe, 2,3, Yurui Gao3,4, Iwona Stepniewska3, Adam Anderson3,4

1Radiology, Vanderbilt University, Nashville, TN, United States; 2Institute of Imaging Science, Vanderbilt University, Nashville, TN, United States; 3Biomedical Engineering, Vanderbilt University; 4Institute of Imaging Science,, Vanderbilt University, Nashville, TN, United States


The light microscopy images have a high spatial resolution and are usually co-registered to the corresponding MR images in order to make the comparison. However, there are artifacts, such as tearing, deformation, or disappearance of tissue fragments, in the stained slices. Those artifacts make the registration among MR, blockface, and light images more difficult. In this study, two image post-processing techniques are introduced, which can provide a better initialization to the nonrigid registration algorithm.