Optimization of 19F MR Quantification of Administrated PFC Nanoparticle in Vivo: Mathematical Simulation and Experimental Validation

Lingzhi Hu¹, Lei Zhang¹, Junjie Chen¹, Gregory M. Lanza¹, Samuel A. Wickline¹

Acquisition parameters for in vivo 19F MR quantification of administrated PFC nanoparticle (NP) have been optimized, in the sense of minimizing the oxygenation effect and maximizing the SNR. Bloch equation based simulation has been performed to simulate the acquired intensity of perfluoro-15-crown-5-ether NP under normoxia and hyperoxia condition. Subsequently, in vivo 19F MR imaging, T1 measurement and 19F quantification have been carried out on C57BL/6 mouse under normoxia and hyperoxia respectively. Both simulation and experiment have validated (1) the optimal TR for achieving high is 1.5*T1; (2) the quantification is insensitive to oxygen level given TR≥ 3* T1.