Zhi-Feng Xu1, Chong-Yang Shen2,
Lin-Ping Wu2, Ye-Yu Xiao, Yao-Wen Chen, Ren-Hua Wu
1medical imging, the 2nd Affiliated
Hospital, the Medical College of Shantou University, shantou, guangdong,
China; 2Multidisciplinary Research Center of Shantou University,
shantou, guangdong, China
In
this study, we attempt to study the alteration of metabolite of MSCs
undergoing adipogenic differentiation to targeted fat cells in vitro, using
9.4T high-resolution 1H NMR spectroscopy.lastly, in our study,several major
metabolites can be observed in the MR sepectroscopy that is before and after
differentiation of MSCsincluding choline, creatine, glutamate and
myo-inositol, acetate and some fatty acids,etc.Quantification of
metabolite concentrations was performedthe levels of intracellular
metabolites, such as choline, creatine, glumate and acetate all decreased,
with the increased level of methionine , succinate and fatty acids after the
MSCs differentiation 2 weeks.It indiactes that we can mintor differentiation
of MSCs,according the changes of metabolites.