Jeremy W. Gordon1, Kang Wang1,
Sean B. Fain1,2, Ian J. Rowland2
1Medical Physics, University of
Wisconsin-Madison, Madison, WI, United States; 2Radiology,
University of Wisconsin-Madison, Madison, WI, United States
This
work describes the successful hyperpolarization of 1,4-13C2
succinate using dynamic nuclear polarization (DNP). By optimizing the
solubility of succinic acid in aqueous solution, levels of solid state similar
to that of pyruvate can be reproducibly obtained. 3.0M solutions of
hyperpolarized succinate were utilized to investigate hepatic metabolism in
vivo. 13C spectra and imaging show that the biodistribution of
succinate within the liver is homogenous. No metabolites were observed in the
time frame of the hyperpolarized experiments. Metabolites were also not
observed in ex vivo organ homogenates.