Li-Wen Lee1,2,
Po-Wah So3, Jimmy D. Bell1
1Metabolic and Molecular Imaging Group,
MRC Clinical Sciences Centre, Hammersmith Hospital Campus, Imperial College
London, London, United Kingdom; 2Department of Diagnostic
Radiology, Chang Gung Memorial Hospital, Chang Gung Univeristy College of
Medicine, Chiayi, Taiwan, Taiwan; 3Preclinical Imaging Unit,
King's College London, Institute of Psychiatry, James Black Centre, Denmark
Hill Campus, London, United Kingdom
Glucose
homeostasis is regulated by β-cell insulin secretion, peripheral glucose
uptake and hepatic glucose production. We applied manganese-enhanced magnetic
resonance imaging (MEMRI), utilising Mn2+ as a surrogate marker
for Ca2+, to assess Ca2+-related processes involved in
glucose homeostasis. MEMRI was performed following i.p. glucose or vehicle
and signal intensity (SI) measured to assess Mn2+ distribution.
MEMRI showed a significant increase in SI in skeletal muscle and the pancreas
following glucose challenge but not in the liver. Thus, MEMRI can be used to
assess changes in skeletal muscle glucose uptake and β-cell insulin
secretion in vivo.