Abdel Wahad Bidar1, Karolina Ploj2,
Christopher Lelliott2, Karin Nelander3, Leonard
Storlien2, Paul Hockings1
1DECS Imaging, AstraZeneca R&D,
In
preclinical drug discovery, experimental rodent models of obesity are used
for the investigation of metabolic disorders. Repeated in vivo measurements
of adipose tissue depots and intraorgan fat can provide longitudinal data
with greatly reduced usage of experimental animals. The aim of the present
study was threefold: (i) validate in vivo MRI/S determinations of brown adipose
tissue, total, intra-abdominal and subcutaneous white adipose tissues as well
as intrahepatocellular lipids against ex vivo measurement, (ii) address the
3Rs mandate, by presenting a statistical power analysis; (iii) characterize
the phenotypic and metabolic switch of the cafeteria-diet mouse model
during a dietary intervention.