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Abstract #0457

In Vivo 3D 19F Fast Spectroscopic Imaging (F-UTSI) of Angiogenesis on Vx-2 Tumors in Rabbits Using Targeted Perfluorocarbon Emulsions

Rolf Lamerichs1, Muhammed Yildirim1,2, Aart J. Nederveen3, Jaap Stoker3, Gregory M. Lanza4, Samuel A. Wickline4, Shelton D. Caruthers4

1Philips Research, Eindhoven, Netherlands; 2Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands; 3Department of Radiology, Academic Medical Center, Amsterdam, Netherlands; 4Washington University, St Louis, MO, United States


Quantitative molecular MRI of angiogenesis using site-targeted 19F agents has great potential. Many 19F agents, however, possess complex spectra with many resonances over a wide ppm range. Fluorine ultra-fast Turbo Spectroscopic Imaging (F-uTSI) has been developed to overcome these drawbacks while offering the advantage of distinguishing various 19F compounds based on chemical shift differences thereby allowing multi-color imaging. Herein, F-uTSI is shown to be an efficient, sensitive technique for quantitatively detecting minute amounts of 19F contrast agents in vivo while overcoming the confounding problems associated with chemical shift. Employing functionalized perfluorocarbon nanoparticles in Vx2 tumor-bearing rabbits, angiogenic maps were created.