Junzhong Xu1, Jingping Xie1,
Jerome Jourquin2, Daniel C. Colvin1, Mark D. Does1,
Vito Quaranta2, John C. Gore1
1Institute of Imaging Science,
Vanderbilt University, Nashville, TN, United States; 2Cancer
Biology, Vanderbilt University, Nashville, TN, United States
Proliferating
tumors usually contain a much higher fraction of cells in active cell
division phases, so for a full understanding of the diffusion properties of
tumors it is necessary to understand the changes that occur in cells in
different phases. Here we report how oscillating gradient spin echo (OGSE)
methods detect intracellular changes of synchronized HL-60 cells at different
phases, while conventional pulsed gradient spin echo (PGSE) methods cannot
distinguish changes at sub-cellular dimensions due to relatively long
diffusion times. This feature means OGSE methods may provide extra contrast
for detecting cancer.