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Abstract #3774

In Vivo Murine Cardiac PCr and ATP Concentrations Measured by Magnetic Resonance Imaging and Spectroscopy

ashish gupta1, V. P. Chacko2, Robert G. Weiss

11Department of Medicine, Division of Cardiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; 2Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA


An in vivo 1H MRI and 31P MR spectroscopic method is proposed and validated for the measurement of in vivo high energy phosphate metabolite (PCr and ATP) concentrations in normal (n=7) and thoracic aorta constriction (TAC) (n=10) mouse hearts. The in vivo MR results for [ATP] are in good agreement with those obtained using an in vitro luminescent assay on perchloric acid extracts of the same hearts.