Nicholas G. Spencer1, Eszter Nagy2, Nandita M. DeSouza1, Geoffrey S. Payne1
1CRUK Clinical Magnetic Resonance Research Group, Institute of Cancer Research and the Royal Marsden Hospital, Sutton, Surrey, UK; 2Human Biomonitoring and Carcinogen Activation, Institute of Cancer Research and the Royal Marsden Hospital, Sutton, Surrey, UK
With the long-term aim to evaluate polyamines as magnetic resonance (MR) markers of androgen-sensitivity in prostate cancer, this study compares polyamine levels in androgen-dependent and androgen-independent cell lines in response to androgen deprivation. An androgen-responsive cell line, 22RV1, showed lower polyamine levels compared to androgen-independent PC-3 cells and, in androgen deprived medium, there was a decrease in polyamines for 22RV1 but not in PC-3 cells. This was confirmed with high performance liquid chromatography and demonstrates the potential of MR in the analysis of polyamine metabolism in-vitro models of prostate cancer.